Long-term effects of cryopreservation on human spermatozoa.
نویسندگان
چکیده
We evaluated the long-term effects of cryopreservation on spermatozoa of men with testicular cancer, lymphoma, and benign conditions. No statistically significant decrease in semen parameters was noted. Duration of follow-up was up to 5 years and mean follow-up was 2 years.
منابع مشابه
The Effects of EGTA on the Quality of Fresh and Cryopreserved-Thawed Human Spermatozoa
Background: Sperm cryopreservation-thawing process has damaging effects on the structure and function of sperm, namely cryoinjury. Calcium overload has been reported as a postulated mechanism for sperm damage during the first steps after thawing. This study was designed to assess the intracellular calcium (Ca2+i) after cryopreservation and to clarify the role of a calcium chelator ethylene glyc...
متن کاملP-12: Effect of Centrifugation on Levels of Stress Oxidative of Human Spermatozoa
Background: Cryopreservation has adverse effect on human spermatozoa via exposure to physical and chemical stress that may lead to alter in reactive oxygen species (ROS) levels. During sperm preparation or cryopreservation, centrifugation as a physical stress may effect on sperm quality probably with stress oxidative production and membrane damage. Therefore the aim of this study was to evaluat...
متن کاملImpact of Cryopreservation Process on Viability, Nitric Oxide and DNA Apoptosis in Fertile Human Spermatozoa
Introduction: Infertility is a common phenomenon in modern societies. Today, use of assisted reproductive technologies (ARTs) for treatment of infertility is common, and cryopreservation is one of these technologies. Cryopreservation has impact on the function and percentage of fertility of human sperm. In this study, motility, viability, nitric oxide, and DNA apoptosis were assessed before and...
متن کاملI-16: Assessment of The Vitrified Ovarian Tissue in Long Term Culture
In vitro culture of human ovarian tissue the following cryopreservation is proposed for follicular development. There are no techniques that guarantee successful maturation of the follicles within the excised tissue. The viability of cultured human ovarian tissue improved by adding some growth factors to the culture media. The efficiency of vitrification as the cryopreservation method for human...
متن کاملNerve growth factor in human semen: Effect of nerve growth factor on the normozoospermic men during cryopreservation process
Objective(s):Althoughroutinely applied in assisted reproductive technology, human sperm cryopreservation is not a completely successful procedure. Adverse effects of cryopreservation on the fertilization capacity, motility, morphology, and viability of spermatozoa have been proven; cryopreservation has also shown a role in sperm DNA fragmentation and infertility. The post-thaw survival of sperm...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Fertility and sterility
دوره 84 2 شماره
صفحات -
تاریخ انتشار 2005